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. Author manuscript; available in PMC: 2017 Mar 22.
Published in final edited form as: Anal Chem. 2016 Sep 27;88(20):10301–10308. doi: 10.1021/acs.analchem.6b03148

Figure 1. The general MSn analysis workflow for identifying and quantifying TMT-labeled, DSSO cross-linked peptides.

Figure 1

Fragment ions from MS2 are selected for subsequent HCD analysis in MS3, releasing both b and y ions for sequencing, as well as TMT reporter ions for quantitation.