Figure 3. Three MSn acquisition methods utilized for analyzing TMT-labeled, DSSO cross-linked peptides.
(A) Method 1: targeted identification method, ID-MS3. (B) Method 2: MultiNotch quantitation using synchronous precursor selection (SPS), SPS-MS3. (C) Method 3: combined acquisition method consisting of ID-MS3 with SPS-MS3, ID-SPS-MS3.