Fig 5. Capillary electropherograms of HP-NAP purified from DEAE negative mode chromatography at pH 7.0 to 9.0.
The recombinant HP-NAP obtained from the unbound fractions of DEAE Sepharose and DEAE Sephadex resins at pH 7.0 to 9.0 was subjected to CE analysis at 25°C as described in Materials and Methods. The UV absorbance was recorded at 220 nm. 4-Methoxybenzyl alcohol (4mBA) was used as the neutral electroosmotic flow (EOF) marker, which migrated as a peak at μe = 0 for all electropherograms. The peaks shown in the electropherograms are HP-NAP (━), 4mBA (—-), and HP-NAP together with 4mBA (⋯). Data were representative of two independent experiments.