Fig 9. Capillary electropherograms of HP-NAP purified by gel-filtration chromatography at pH 8.5 and 9.0.
HP-NAP purified by gel-filtration chromatography at pH 9.0 (25°C) was kept at pH 9.0 or adjusted to pH 8.5 and then diluted to a protein concentration of 0.1 mg/ml. The CE analysis was performed at 25°C with UV absorbance recorded at 220 nm as described in Materials and Methods. The peaks shown in the electropherograms are HP-NAP (━), 4mBA (—-), and HP-NAP together with 4mBA (⋯). 4mBA, as the neutral EOF marker, was shown as a peak at μe = 0 for all electropherograms. Data were representative of two independent experiments.