Table 1. The proposed surface charge and the calculated electrophoretic mobility of HP-NAP purified by DEAE negative mode chromatography and gel-filtration chromatography at pH 7.0 to 9.0 based on the CE data.

	HP-NAP surface charge; HP-NAP electrophoretic mobility (m2/V/s) a
pH	DEAE negative mode chromatography b		Gel-filtration chromatography c
	DEAE sepharose	DEAE sephadex
7.0	• neutral d	• neutral d	NA e
7.5	• neutral d	• neutral e	NA e
8.0	• neutral d	• neutral d	NA e
8.5	• neutral f • negative;–(5.06 ± 0.03) x 10−9	• neutral f • negative;–(6.02 ± 0.09) x 10−9	• negative;–(5.36 ± 0.03) x 10−9
9.0	• neutral f • negative;–(7.47 ± 0.02) x 10−9	• neutral f • negative;–(7.40 ± 0.08) x 10−9	• negative;–(7.10 ± 0.10) x 10−9

^a Only the electrophoretic mobility of the negative species is shown.

^b The electropherograms of the CE experiments are shown in Fig 5.

^c The electropherograms of the CE experiments are shown in Fig 9.

^d All HP-NAP co-migrated with the neutral EOF marker, 4-methoxybenzyl alcohol as shown in Fig 5.

^e NA: not available. No peak was detected in the CE experiments (data not shown).

^f Some HP-NAP co-migrated with the neutral EOF marker, 4-methoxybenzyl alcohol as shown in Fig 5.