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. Author manuscript; available in PMC: 2017 Mar 22.

Published in final edited form as: Blood. 2011 Oct 19;118(26):e192–e208. doi: 10.1182/blood-2011-04-345330

Quantitative PCR for (A) genes most differentially expressed in rM versus pro-inflammatory macrophages validating original microarray findings including (B) CD209a, the monocytes-derive DC marker, which was included arising from the DC-like phenotype deduced in Figure 3. For comparisons to established M1/M2 cells, BMDMs were incubated with either LPS/INFγ (M1) and or IL-4 (M2) for 24h. RNA was extracted and probed for a range of typical (C) M1, (D) M2, and (E) M2b markers. Data are represented and analysed by ANOVA followed by Bonferroni multiple comparison tests. Values are expressed as means ± SEM of n=5-6 mice per group. ** P value < 0.01 and *** P value < 0.001.

Quantitative PCR for (A) genes most differentially expressed in rM versus pro-inflammatory macrophages validating original microarray findings including (B) CD209a, the monocytes-derive DC marker, which was included arising from the DC-like phenotype deduced in Figure 3. For comparisons to established M1/M2 cells, BMDMs were incubated with either LPS/INFγ (M1) and or IL-4 (M2) for 24h. RNA was extracted and probed for a range of typical (C) M1, (D) M2, and (E) M2b markers. Data are represented and analysed by ANOVA followed by Bonferroni multiple comparison tests. Values are expressed as means ± SEM of n=5-6 mice per group. ** P value < 0.01 and *** P value < 0.001.