Figure 4.

Env mutations, antibody binding phenotypes, and the phylogeny representing CH505 sequences selected for immunological testing. (A) Accruing mutations among a subset of 32 LASSIE selected gp120 sites are indicated as colored boxes. Blanks match the TF, red and blue depict negative and positive charge change, black shows insertions/deletions, cyan a gain of an asparagine in a potential N‐linked glycosylation motif, and gray shows other mutations. (B) Log AUCs of ELISA binding are displayed as heatmaps to show the relative affinities for the CH235 and CH103 bNAb lineage members, one column per antibody and one row per Env. Deeper reds indicate higher affinity, gray undetected. Each row in this figure follows the ordering of leaves in the tree on the right (C), which was made by maximum likelihood (in phyML, with HIVw+g4+I) from gp120 sequences. The CH505 TF sequence was used to root the tree. Note that these sequences were selected for experimental evaluation prior to the availability of LASSIE. The six lineage‐based Envs [ref. 68] are outlined