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. Author manuscript; available in PMC: 2018 Jan 19.
Published in final edited form as: Cell Chem Biol. 2017 Jan 5;24(1):87–97. doi: 10.1016/j.chembiol.2016.12.007

Figure 3. APT2 inhibition enhances Scrib perimeter localization in Snail-transformed cells.

Figure 3

(A–B) Representative immunofluorescence images of Scrib in MDCK-control and MDCK-Snail cells treated overnight with indicated inhibitors. (C) Dose-dependent enhancement of Scrib perimeter localization quantified by high-content automated microscopy, and processed as described in the Supporting Experimental Procedures. ML349-inactive lacks the two sulfone oxygens, which are required for APT2 inhibition in vitro. Standard deviations are shown for each condition. (D) APT2 knockdown in MDCK-Snail rescues Scrib membrane-localization. Representative images from high-content analysis of MDCK Control and Snail lines transfected with either non-targeting (siControl) or APT2 siRNA. Scale bar = 40 μm across each panel.