Fig. 3.

Oxidative stress response in E. coli follows the expression of eGFP. Oxidative stress response was determined by the quantification of mRNAs by real-time PCR of genes activated by the SoxR/S and OxyR regulons in E. coli. (a) eGFP expression (first three bars on the left) and quantification of genes that are targets for the activation of the SoxR/S regulon are shown. (b) Quantification of gene expression for different genes activated by OxyR. (c) Quantification of mRNAs for MBP (maltose binding protein) and targets of SoxR/S and OxyR were determined in an E. coli strain engineered for the controlled expression of high levels of MBP. * is used to show statistically significant differences between samples and controls, and $ shows statistically significant differences between samples of high and low expression.