Scheme 1.
GFP chromophore maturation and proposed mechanism for the catalytical consumption of NAD(P)H and generation of O2•–/H2O2 by GFP immature intermediates. The GFP chromophore maturation process is shown in black (adapted from Barondeau et al. [28]). Shown in red, the proposed reaction of the last immature intermediate of GFP with NAD(P)H leads to the catalytical generation of O2•–/H2O2 and consumption of NAD(P)H. The eGFP structure is shown (PDB# 2y0g).