Fig. 1.

Experimental Design. (A) Subjects (n=10) were randomized to have remote ischemic conditioning (RIC) applied to their forearm (n=5) or thigh (n=5) followed by a 2 week washout period before RIC was applied to the other extremity. (B) The study consisted of a baseline period followed by RIC (4 cycles of 5 min cuff inflation to induce ischemia and 5 min reperfusion following rapid deflation). Blood flow (LSCI) and tissue oximetry (Near Infrared Spectroscopy) measurements were begun 1 min prior to RIC initiation and ended 5 min after the last RIC cycle. Blood was obtained at baseline, 4 min into reperfusion (i.e. 1 min before next cycle) and at 24 h.