Fig. 4.

RIC inhibits platelet mitochondrial respiration. (A) Representative Seahorse XF Analysis trace measuring oxygen consumption rate (OCR) in platelets from one subject at baseline (gray circles), after 4 cycles of RIC applied to the arm (open squares), and 24 h later (black triangles). For each trace, after a baseline reading, oligomycin A (2.5 µmol/L), FCCP (0.7 µmol/L) and rotenone (15 µmol/L) were added as noted by the arrows. (B-C) Quantitation of (B) basal and (C) maximal respiration rates calculated from traces similar to that shown in (A). (D) Mitochondrial superoxide production by platelets at baseline and after 4 cycles of RIC applied to the arm or thigh. (B–D) are means±SEM. *p<0.01 compared to baseline for the respective appendage. N=10.