Table 1. The progression of BU signs in the various treatment groups post-inoculation.
| Experiments | Earliest time (days) of visible signs post-inoculation (respondents/number of mice) | AFB in fine needle aspirate (FNA) from ulcer | M. ulcerans culture from FNA and from ulcer | ||
|---|---|---|---|---|---|
| Infection by topical application of inoculum on punctured skin | Inflammation | Edema | Ulcer | ||
| 50 μl of M. ulcerans (2.25 x 106 CFU/ml), positive control (Group 1, n = 3) | 31 (3/3) | 45 (3/3) | 49 (3/3) | Yes (3/3) | Yes (3/3) |
| 50 μl of A. polyphaga (1.6 x 106 cells/ml) infected with M. ulcerans (2.96 x 104 CFU / ml) (Group 2, n = 3) | 31 (3/3) | 44 (3/3) | 49 (3/3) | Yes (3/3) | Yes (3/3) |
| 50 μl of A. polyphaga trophozoites (1.6 x 106 cells/ml) (Group 3, n = 3) | None (3/3) | None | None | Not applicable (N/A) | N/A |
| 50 μl of PYG (Group 4, n = 3) | None (3/3) | None | None | N/A | N/A |
| 50 μl of distilled water (Group 5, n = 3) | None (3/3) | None | None | N/A | N/A |
| Infection by injection into footpad | |||||
| 50 μl of M. ulcerans (2.25 x 106 CFU / ml,) positive control (Group 4, n = 3) | 1 (3/3) | 1 (3/3) | 21 (3/3) | Yes (3/3) | Yes (3/3) |
| 50 μl of A. polyphaga (1.6 x 106 cells/ml) infected with M. ulcerans (2.96 x 104 CFU / ml)) (Group 5, n = 3) | 3 (3/3)* | 7 (3/3) | 25 (3/3) | Yes (3/3) | Yes (3/3) |
| 50 μl of M. ulcerans (2.96 x 104 CFU / ml) (Group 6, n = 3) | 14 (3/3) | 31 (3/3) | 51 (3/3) | Yes (3/3) | Yes (3/3) |
| 50 μl of PYG (Group 4, n = 3) | None (3/3) | None | None | N/A | N/A |
| 50 μl of distilled water (Group 5, n = 3) | None (3/3) | None | None | N/A | N/A |
AFB = Acid fast bacilli, FNA = Fine needle aspirate