Skip to main content
. 2017 Apr 1;8(2):228–239. doi: 10.14336/AD.2016.0910

Figure 5.

Figure 5.

WtP53 but not Δ40p53 and Δ133p53 stabilizes the interaction between APE and AP-DNA containing whole-mitochondrial extracts. (A) Δ133p53 attenuated APE-interaction with AP-DNA (lanes 7 and 8); and Δ40p53 weakly stimulated APE interaction with AP-DNA (lanes 9 and 10). (B) d4T does not affect APE interaction with AP-DNA. p53 can interact with the APE-AP-DNA complex (lanes 9-11) but Δ40p53 and Δ133p53 cannot interact with the APE-AP-DNA complex (lanes 3-8). (C) A representative immunoprecipitation and Western blot from different p53 proteins stabilizing the interaction between APE and AP-DNA containing oligos (A and B). The data show that only wtP53 interacts with APE1 or DNA pol-γ.