TABLE 1.

Metadata and 1D sequencing run performance for the Antarctic tests

Sample	Description	Sequencing date	Ambient T	Conditions	Sequencing location	Total # reads	Pass/fail ratio	Longest pass read, bp	Mean pass read length, bp	Median pass read length, bp	Mean Q score	Duration of run
Control	lambda Phage DNA	Nov. 23, 2016	RT	S. S. Johnson lab	Georgetown University	6100	0.761	79,414	7763	4910	8.1	3 h
Control	lambda Phage DNA	Feb. 24, 2017	4°C	S. S. Johnson lab	Georgetown University	4525	0.021	15,922	3764	2178	8.1	50 min
Calibration	Configuration cell	Dec. 10, 2016	−5°C	Light snow, overcast	Hut Point	N/A	N/A	N/A	N/A	N/A	N/A	N/A
Environmental	Paleomat, Lake Fryxell	Dec. 15, 2016	RT	Crary lab	McMurdo Station	46,936	0.121	90,183	562	301	5.7	10 h
Environmental	Modern mat, Lake Vida	Dec. 17, 2016	RT	Crary lab	McMurdo Station	18,761	0.272	171,106	3874	2067	7.8	8 h
Environmental	Paleomat, Lake Vanda	Dec. 19, 2016	−1°C	Sunny, slight wind	Taylor Valley	6026	0.035	22,128	777	484	5.1	50 mina
Environmental	Paleomat, Lake Vida	Dec. 20, 2016	2°C	Sunny	Taylor Valley	573	0.139	21,357	3449	1238	4.6	2.5 h

^aRun terminated for evacuation in advance of impeding weather.