Fig 4. Immunofluorescence staining using monoclonal antibody to detect HEV antigen in the liver from cynomolgus monkeys obtained at the end of the experiment.

(A) Negative immunofluorescence of monkey from G1 after viral clearance. (B) Detection of HEV antigen (labelled in green) in sinusoidal lining cells of chronically infected monkey from G2. (C) Negative results in control monkey from G3. Cell nuclei were counterstained with DAPI (blue).