Fig 1. Growth assays.
F. tularensis (A) or F. novicida (B) strains were grown in CDM at 37°C with (empty) or without (filled) the presence of A5P at a concentration of 400 μM. Growth was monitored by optical density. OD measurements were based upon quadruplicate samples and bars represent standard error of the mean. These data represent at least two separate experiments. The F. tularenesis kdsD::ltrBL1 and F. novicida kpsF::T20 mutants were severely altered for growth in Chamberlain’s medium. However, the addition of A5P to the medium significantly increased the growth of these strains. In contrast, the presence of A5P did not affect the growth of the F. tularensis CipR mutant as the two lines completely overlapped.