Table 2. Oligonucleotides used in this study.
Oligonucleotide | Sequence |
---|---|
611|612s-IBS | AAAACTCGAGATAATTATCCTTAGCATGCCCGCTAGTGCGCCCAGATAGGGTG |
611|612s-EBS1d | CAGATTGTACAAATGTGGTGATAACAGATAAGTCCCGCTAAATAACTTACCTTTCTTTGT |
611|612s-EBS2 | TGAACGCAAGTTTCTAATTTCGATTCATGCTCGATAGAGGAAAGTGTCT |
kdsD 5' cloning | CGGACCGGATTAATTTGAATATGTTTCAT |
kdsD 3' cloning | CGGACCGGTTAGGTGATCCTGTAATGCTTA |
Kan probe F | TGCATGGTTACTCACCACTGC |
Kan probe R | TACAACCTATTAATTTCCCCTCG |
Bolded sequence corresponds to XhoI restriction enzyme site. Underlined sequence corresponds to BsrGI restriction site. Italics sequence corresponds to RsrII restriction enzyme site.