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. 2017 Mar 22;12(3):e0174106. doi: 10.1371/journal.pone.0174106

Table 2. Oligonucleotides used in this study.

Oligonucleotide Sequence
611|612s-IBS AAAACTCGAGATAATTATCCTTAGCATGCCCGCTAGTGCGCCCAGATAGGGTG
611|612s-EBS1d CAGATTGTACAAATGTGGTGATAACAGATAAGTCCCGCTAAATAACTTACCTTTCTTTGT
611|612s-EBS2 TGAACGCAAGTTTCTAATTTCGATTCATGCTCGATAGAGGAAAGTGTCT
kdsD 5' cloning CGGACCGGATTAATTTGAATATGTTTCAT
kdsD 3' cloning CGGACCGGTTAGGTGATCCTGTAATGCTTA
Kan probe F TGCATGGTTACTCACCACTGC
Kan probe R TACAACCTATTAATTTCCCCTCG

Bolded sequence corresponds to XhoI restriction enzyme site. Underlined sequence corresponds to BsrGI restriction site. Italics sequence corresponds to RsrII restriction enzyme site.