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. 2017 Mar 22;12(3):e0174106. doi: 10.1371/journal.pone.0174106

Table 5. Susceptibilities of F. tularensis and F. novicida to hydrophobic agents.

Strain PMB ± SD1 Tween 20 ± SD Triton X ± SD SDS ± SD
Ft Schu S4 10.0 ± 0 18.1 ± 1.06 35.2 ± 3.01 34.1 ± 8.44
Ft CipR 13.7±3.22 32.5 ± 5.85* 35.2 ± 4.27 36.97±6.51
Ft kdsD::ltrBL1 11.6 ± 0.2.718 24.9 ± 0.93* 39.1 ± 0.98 52.20 ± 1.57
Ft kdsD Comp 10.0 ± 0 18.7 ± 2.00 36.3 ± 1.12 32.0 ± 8.39
Fn U112 19.2 ± 2.54 10.0 ± 0 31.7 ± 2.51 23.0 ± 2.00
Fn kpsF::T20 20 ± 0.89 19.7 ± 1.96* 29.6 ± 0.51 29.1 ± 0.56*
Fn kpsF Comp 20.2 ± 0.40 10.0 ± 0 28.8 ± 6.75 24.8 ± 1.57

Sterile paper disks (10 mm in diameter) were saturated in polymyxin B at 10 mg/ ml, Tween 20 at 5%, Triton X-100 at 5%, or SDS at 100 mg/ ml, dried, and placed in triplicate onto separate agar plates. Sensitivity to each agent was assessed by measuring the diameter of the zone of growth inhibition around the disk. The results are in millimeters and the average of the multiple measurements of disks from three separate experiments.

Those inhibitors which displayed significant differences (p< 0.05) with the mutants strain as compared to measurements with the respective parent strain are indicated by *.

1 ± SD (standard deviation)