Table 5. Susceptibilities of F. tularensis and F. novicida to hydrophobic agents.
Strain | PMB ± SD1 | Tween 20 ± SD | Triton X ± SD | SDS ± SD |
---|---|---|---|---|
Ft Schu S4 | 10.0 ± 0 | 18.1 ± 1.06 | 35.2 ± 3.01 | 34.1 ± 8.44 |
Ft CipR | 13.7±3.22 | 32.5 ± 5.85* | 35.2 ± 4.27 | 36.97±6.51 |
Ft kdsD::ltrBL1 | 11.6 ± 0.2.718 | 24.9 ± 0.93* | 39.1 ± 0.98 | 52.20 ± 1.57 |
Ft kdsD Comp | 10.0 ± 0 | 18.7 ± 2.00 | 36.3 ± 1.12 | 32.0 ± 8.39 |
Fn U112 | 19.2 ± 2.54 | 10.0 ± 0 | 31.7 ± 2.51 | 23.0 ± 2.00 |
Fn kpsF::T20 | 20 ± 0.89 | 19.7 ± 1.96* | 29.6 ± 0.51 | 29.1 ± 0.56* |
Fn kpsF Comp | 20.2 ± 0.40 | 10.0 ± 0 | 28.8 ± 6.75 | 24.8 ± 1.57 |
Sterile paper disks (10 mm in diameter) were saturated in polymyxin B at 10 mg/ ml, Tween 20 at 5%, Triton X-100 at 5%, or SDS at 100 mg/ ml, dried, and placed in triplicate onto separate agar plates. Sensitivity to each agent was assessed by measuring the diameter of the zone of growth inhibition around the disk. The results are in millimeters and the average of the multiple measurements of disks from three separate experiments.
Those inhibitors which displayed significant differences (p< 0.05) with the mutants strain as compared to measurements with the respective parent strain are indicated by *.
1 ± SD (standard deviation)