Fig 4. Labeled cells of renin lineage (CoRL) co-express podocin in glomeruli of Ren1cCre /R26R-ConfettiTG/WT mice with experimental FSGS.

(A¹-A⁷) At baseline: (A¹) Confocal image shows podocin antibody staining (magenta). (A²-A⁵) All 4 CoRL reporters (green, red, blue, yellow) can be detected without the use of antibody. (A⁶) Composite image of all 4 reporters and podocin staining. (A⁷) For ease of viewing, all 4 confetti reporter channels have been converted to green, and podocin has been converted to red, so that co-localization can be visualized as yellow. All four confetti reporters are seen in the JGC, with no overlap with podocin staining. (B¹-B⁷) At day 14 FSGS: (B¹) There is a segmental decrease in podocin staining in the left lower quadrant of the glomerular tuft. (B²-B⁶) Multi-clonal CoRL (red, yellow and green) are detected in glomerular tuft, but do not co-localize with podocin. (B⁷) The CoRL reporters in the tuft do not co-localize with podocin staining. (C¹-C⁷) At D28 FSGS: (C¹-C⁵) Podocin and multi-clonal CoRL (red, yellow and green) are detected in the glomerular tuft. (C⁶) Composite image of all four reporters and podocin staining. (C⁷) CoRL reporters co-localize with podocin, creating a yellow color (arrows indicate examples).