The confocal images in the left column (A-C) represent nephrin staining (magenta) detected by antibody, and 4 CoRL reporters (green, red, blue, yellow) detected without antibody. The confocal images in the right column represents the same image on the left, but for ease of viewing, all 4 confetti reporter channels have been converted to green, and nephrin has been converted to red, so that co-localization can be visualized as yellow. (A, B) At baseline: All four CoRL reporter colors are restricted to the JGC (dashed white box), and nephrin staining is restricted to the glomerular tuft. (B) all four Confetti CoRL reporters (green) are seen in the JGC, with no overlap with nephrin (red). (C, D) At D14 FSGS: (C) There is a segmental decrease in nephrin staining in the right upper quadrant of the glomerular tuft. Multi-clonal CoRL are detected in glomerular tuft, but do not co-localize with nephrin. (D) The CoRL reporters in the tuft do not co-localize with nephrin staining. (E, F) At D28 FSGS: (E) Multi-clonal CoRL are detected in the glomerular tuft. (F) CoRL reporters co-localize with nephrin, creating a yellow color (arrows indicate examples).