Table 1. Primers used for amplification of 3’UTR C/EBPβ mRNA.
3’UTR of C/EBPβ mRNA | Forward primer 5’-3’ | Reverse primer 5’-3’ |
---|---|---|
-60-624 | ttactcgagctgcggaacttgttcaagcag | attgtcgactttttttactgcccccaaaaggc |
15–624 | attctcgagcgtccccctgccggcc | attgtcgactttttttactgcccccaaaaggc |
15–270 | attctcgagcgtccccctgccggcc | attgtcgacggcagagggagaagcagaga |
268–450 | attctcgagtctctgcttctccctctgcc | attgtcgaccatcaacagcaacaagcc |
432–624 | attctcgagggcttgttgctgttgatg | attgtcgactttttttactgcccccaaaaggc |
Primers sequence design was done on the basis of cDNA (NM_005194.3) sequence.