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. 2017 Mar 8;13(3):e1006658. doi: 10.1371/journal.pgen.1006658

Fig 3. DUX4 induces nuclear dsRNA accumulation and phosphorylation of EIF2AK2/PKR and EIF2S1/eIF-2α.

Fig 3

(A) Fluorescence microscopy using the J2 antibody to detect dsRNA in RD-DUX4i cells treated with doxycyline for 16 hours or transfected with poly(I:C). Note that not all nuclei showed dsRNA aggregation, likely due to asynchronous activation of the DUX4 transgene. (B) Western blot of phosphorylated and unphosphorylated PKR in extracts from RD-DUX4i cells treated with doxycyline for 21 hours or transfected with poly(I:C). (C) Western blot in extracts from RD-DUX4i cells treated with doxycyline for the indicated times and using the indicated antibodies.