Table 1. Top 16 filtered targets with Z-score > 3.0 from RD-DUX4i siRNA screen.
| Target ID | DUX4i Z-score | LUCi Z-score | AVG FPKMa-DOX | AVG FPKMa +DOX | Validation % of -DOXb | Mean normalized Fold Rescuec |
|---|---|---|---|---|---|---|
| FOSB | 3.86 | 0.66 | 0.16 | 8.59 | 54.7 | 75.17 |
| RNASEL | 3.71 | -0.07 | 0.94 | 0.54 | 52.4 | 63.50 |
| MYC | 3.69 | -0.29 | 29.87 | 696.89 | 63.9 | 62.60 |
| FXN | 3.67 | -0.06 | 3.51 | 0.77 | 61.6 | 61.70 |
| EAF1 | 3.63 | -0.32 | 5.89 | 19.27 | 34.0 | 58.60 |
| PHF20 | 3.39 | 0.77 | 4.93 | 1.36 | 38.3 | 44.93 |
| NLGN2 | 3.36 | -0.09 | 8.85 | 1.77 | NA | 43.17 |
| TGS1 | 3.35 | 0.78 | 10.11 | 12.56 | NA | 43.10 |
| HSD17B4 | 3.29 | 0.92 | 5.57 | 2.09 | NA | 39.97 |
| ITPK1 | 3.28 | 1.1 | 1.41 | 0.44 | NA | 39.60 |
| SGK196 | 3.19 | 0.23 | 4.64 | 2.52 | NA | 36.47 |
| CDC20 | 3.15 | -0.41 | 79.51 | 358.73 | NA | 33.87 |
| QSOX1 | 3.15 | 0.19 | 7.54 | 4.37 | NA | 33.87 |
| THOP1 | 3.06 | 0.1 | 11.19 | 3.34 | NA | 30.87 |
| FBN2 | 3.04 | -0.08 | 0.91 | 0.51 | NA | 30.37 |
| APH1A | 3.02 | -0.33 | 15.96 | 4.79 | NA | 30.00 |
aAVG FPKM, mean fragments per kilobase of transcript per million mapped reads across three replicates.
bValidation % of -DOX, mean CellTiter-Glo determined viability of the indicated target as a percentage of the no doxycycline control in the validation experiment as in S3A Fig. 'NA' indicates that the given target was not retested. For comparison purposes, the scrambled control siRNA viability was 4.7% of the -DOX control.
cMean Normalized Fold Rescue, the average ratio between raw target fluorescent measurements from the original screen and the sample median for the 384-well plate as a whole. For comparison purposes, the average ratio for the more robust DUX4 siRNA was 80.0.