Video 10. Motility of tethered CatSperz +/- and -/- sperm after A23187 treatment; 30 min after wash, related to Figure 6—figure supplement 1.
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DOI: 10.7554/eLife.23082.030
Spermatozoa treated with 20 µM A23187 in H-HTF for 10 min were washed and incubated in HTF under capacitating conditions for 30 min; sperm were tethered to a fibronectin-coated glass bottom dish and the motility was recorded in H-HTF within the next 5 min at 37°C; video rate 100 fps (1/2 speed), 1 s movies. (A) CatSperz+/- and (B) CatSperz-/- spermatozoa.