Figure 4. A V32A point mutation in βC1 enhanced CLCuMuV infection.
(A) CLCuMuB mutant (βV32A), which encodes a mutant βC1V32A, caused the enhanced viral symptom compared to wild type CLCuMuB (β) when co-infected with CLCuMuV (CA). The pictures were taken at 12 dpi. A V32A point mutation in βC1 (βC1V32A) eliminates its interaction with NbATG8f. (B) The incidence of symptom appearance at different time points of post infection. Symptom was indicated as the appearance of curled leaf caused by the infection with CA+β or CA+βV32A. (C) Relative viral accumulation of CLCuMuV DNA. Real-time PCR analysis of V1 gene from CLCuMuV was used to determine viral DNA level. Values represent means ± SE from three independent experiments. (*) p<0.05.