Figure 3. Diastolic failure in sandman mutants.
(A) Representative YT kymographs of 50-day-old animals. Scale bars: (black vertical) 75 µm, (blue horizontal) 1 s. (B) Median heartbeat per genotype at 50 days of age. ⊕, clean excision of the mutagenic piggyBac insertion e00867. (C) Two-dimensional probability map of fractional shortening and systolic interval at 50 days of age. See also Video 2.
DOI: http://dx.doi.org/10.7554/eLife.20851.008
Figure 3—source data 1. Median heartbeats for all individual animals in panel B.
Median heartbeats were calculated for individual animals (Table) and for all consolidated heartbeats for a respective genotype (Panel B and last column of each Table). This source data provides a representation of the observed heartbeat waveform variability between animals.
elife-20851-fig3-data1.xlsx (609.3KB, xlsx)
DOI: 10.7554/eLife.20851.009
Figure 3—figure supplement 1. sandman and galene genetic loci.
(A,B) Genomic maps of the sandman and galene loci, adapted from the UCSC genome browser (https://genome.ucsc.edu), with a sequence conservation index across a panel of 15 insects. The piggybac insertions used to generate the deletion of sandman are detailed as is the dsRNA sequence used to knockdown galene. The gene CG8712 encodes a protein of unknown function and is reported to be predominantly expressed in the sex organs (http://flybase.org/reports/FBgn0033258.html). (C) Heart-specific RT-PCR for sandman and galene transcripts.
Figure 3—figure supplement 2. RNAi knockdown of galene from birth through 40 days of age.
(A) Two-dimensional probability map of fractional shortening and systolic interval. (B) Estimated cardiac output per second (boxplot) and per stroke (filled circles, mean +/− s.d.). (C) Systolic interval. (D) Median heartbeat. Df, Deficiency BSC431 covering the galene locus. ∅, experimental controls lacking one or the other element of the Gal4-UAS system.