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. Author manuscript; available in PMC: 2017 Dec 4.
Published in final edited form as: J Mol Biol. 2016 Oct 27;428(24 Pt B):4981–4992. doi: 10.1016/j.jmb.2016.10.025

Figure 5. Structural Analysis of LRH-1 - mOct4 Complex.

Figure 5

(a) Overall structure of LRH-1 DBD (orange) bound to the 12 bp Oct4 DR0 (gray) with zinc atoms as spheres. The Oct4 DR0 sequence is shown below with arrows denoting the footprint and orientation of the LRH-1 binding site. (b) LRH-1 makes base-specific contacts with the DR0 (bases in white) mediated by hydrogen bonds (red) made between Glu104, Lys107, Lys 111, Arg112. Mesh shows 2Fo-Fc electron density map contoured to 2σ around the DNA bases. (c) Schematic view of protein-DNA interactions. Larger, bold side chains denoted base-contacting side chains. Water molecules are indicated as red spheres.