Fig. 2.

SDS PAGE and Western blots of Mohave rattlesnake venoms. A.) SDS electrophoresis of Mohave rattlesnake venoms. A total of 25 µg of venom protein was run on a Novex® 10–20% Tricine gel using an XCell SureLock® Mini-Cell for 90 min at 125 V. Lane 1: 1049, lane 2: 1043, lane 3: 252, lane 4: 983, lane 5: 988, lane 6: 990, lane 7: 993, lane 8: 996, lane 9: 997. B.) Western blot of Mohave venoms reacting with the ARDPA. Two identical gels were run, and one was used to transfer proteins onto a nitrocellulose membrane using BioRad Trans-Blot SD Semi-Dry Transfer Cell at 100 V for 1 h and then left overnight. Lane 1: 1049, lane 2: 1043, lane 3: 252, lane 4: 983, lane 5: 988, lane 6: 990, lane 7: 993, lane 8: 996, lane 9: 997. C.) N-terminal sequences of selected protein bands recognized by the ARDPA. Identical gels were obtained, and one was utilized for Western blot while the other one was used for a protein transfer blot. The transfer protein bands, which corresponded to those protein bands recognized by the ARDPA, were excised from the blot and sent for N-terminal sequencing. The bands used are represented by the dashed boxes on the Western blot. Protein classification was determined by the NIH Basic Logical Alignment Search Tool for proteins (BLASTp; https://blast.ncbi.nlm.nih.gov).