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. 2017 Feb 4;8(9):14283–14293. doi: 10.18632/oncotarget.15093

Figure 3. HuR is required for AngII-induced MMP2 and MMP9 expression.

Figure 3

A. Pull-down assay from MOVAS cells homogenates treated for 24 hours with either vehicle or with AngII (1 μM), which were immunoprecipitated with anti-HuR or with IgG. The RNA-bound by HuR was harvested and subjected to real-time PCR by using either MMP2- or MMP9-specific primers. Amounts of input RNA added to the immunoprecipitation reaction mixture was normalized by assessment of GAPDH levels from input RNA by real-time PCR (not shown). Data are depicted as -fold induction compared with vehicle treated cells. B. MOVAS cells were transfected with control or HuR-specific siRNA for 48 hours, followed by AngII treatment and western blot analysis. C. and D. Quantitative analysis of MMP2 and MMP9 protein (C) and mRNAs (D) levels in SMCs (n = 4). *p < 0.05 vs CTR siRNA+vehicle group; #p < 0.05 vs CTR siRNA+AngII group.