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(A) Gelatin zymogram of purified proMMP2 by a two-step affinity chromatography protocol. (B) TIMP2 is not present in the purified proMMP2 preparation, as shown by Western blotting. Positive controls 1 and 2 are supernatants from MDA-MB-231 and T47D cells, respectively. (C) Activation of purified proMMP2 by KLK6 shows the same pattern as in SH-SY5Y supernatants. The experiments were carried out three times.
