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. 2017 Jan 19;8(9):14655–14665. doi: 10.18632/oncotarget.14738

Figure 2. miR-29b functions as a tumor suppressor in cervical cancer.

Figure 2

(A) RT-PCR was performed to detect the expression of miR-29b in cervical cancer cells upon transfection with miR-29b mimic; (B) CCK-8 assays were performed to analyze the effect of miR-29b on cell proliferation of both cell lines. Overexpression of miR-29b suppressed the cell proliferation of both cell lines; (C) Cell cycle analysis, by fluorescence-activated cell sorting (FACS) at 48h after transfection, were performed to analyze the effect of miR-29b on cell cycle progression of both cell lines; (D, E) The effects of miR-29b on cell migration and invasion were detected using trans-well chamber assays. Panel D showed the results on migration (×400); Panel E showed the results on invasion (×400). The data are representative of three independent experiments. Error bars represent s.e.m. *P < 0.05; **P < 0.01.