Skip to main content
. 2017 Jan 18;8(9):14748–14758. doi: 10.18632/oncotarget.14714

Figure 4. Western blot analysis on the expression of HDAC-related proteins.

Figure 4

(A) Expression levels of acetylated histones H3 in various cancer cell lines and the hepatic L02 cells. (B) and (C) Expression levels of acetylated histones H3 and H4, and p21 proteins in HT-29 cells treated with various concentrations of CA-PZ (3.125 μM, 6.25 μM, 12.5 μM, 25 μM, 50 μM, and 100 μM) for 12 h. As shown, the expression levels of Ac-H3, Ac-H4, and p21 proteins were increased. Notably, tCA and the known HDAC inhibitor TSA (1 μM) caused similar changes. However, NR (100 μM) caused less change. (D) Expression levels of acetylated histones H3 and H4, and p21 proteins in MIA PaCa-2 cells treated with various concentrations of CA-PZ. Similar changes as those in HT-29 cells were found.