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. 2017 Jan 27;8(9):14777–14793. doi: 10.18632/oncotarget.14833

Figure 4. E2F1 downregulated miR-519d directly, and miR-519d downregulated E2F1 directly.

Figure 4

(A) Overexpression of E2F-1 represses miR-519d mRNA expression. (B) Silencing of E2F-1 promotes miR-519d mRNA expression. (C) Luciferase reporter assays confirm that miR-519d is the target of E2F-1. (D) The microRNA.org website predicted that the E2F1 mRNA 3′ untranslated region (3′ UTR) contained miR-519d binding sites. (E) Overexpression of miR-519d represses E2F-1 mRNA expression. (F) Luciferase reporter assays confirm that E2F-1 is the target of miR-519d. *p < 0.05.