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. 2017 Jan 27;8(9):14777–14793. doi: 10.18632/oncotarget.14833

Figure 5. MiR-519d inhibits ovarian carcinoma and downregulates RhoC directly.

Figure 5

(A) miR-519d transfection in A2780 and OVCAR3 cells downregulated RhoC, Bcl-2, cyclin D1, survivin, MMP2, MMP9, STAT3, and HuR expression. No changes were observed in p53 expression. (B) The sequence in the 3′UTR of RhoC complementary to the miRNA-519d seed region suggests that RhoC is a direct target of miR-519d. (C) miR-519d transfection reduces mRNA expression of RhoC. (D) Dual luciferase reporter assay indicated that miR-519d directly targets RhoC by binding its 3′UTR. Nude mice injected with cells stably expressing miR-519d showed a (E) dramatic reduction in tumor size and tumor xenograft (F) growth and (G) volumn. (H) miR-519d overexpression downregulates E2F1 and RhoC expression in tumor xenografts in vivo. *p < 0.05.