Skip to main content
. 2017 Jan 20;8(9):15230–15241. doi: 10.18632/oncotarget.14774

Figure 4. Plumbagin inhibits Angiopoietins through inactivation of p-AKT, AKT1, p-PI3K, PI3K, VEGFR1, VEGFR2, p-vegfr1, p-VEGFR2 and activation of p-TIE2 and TIE2.

Figure 4

After co-culture, the hy926 cells were exposed to different concentrations of plumbagin for 48 h. (A) Plumbagin inhibits protein expression. (B–K) The expression of p-AKT, AKT1, p-PI3K, PI3K, p-TIE2, TIE2, VEGFR1, VEGFR2, p-vegfr1, p-VEGFR2 proteins in the cells was analyzed by western blotting using specific antibodies. Thalidomide (25 μM) was used as the positive control, GSK690693 (10 μM) was used as the Akt Inhibitor group. The data represent the mean values of three experiments ± SE.*P < 0.05, **P < 0.01, ***p < 0.001 compared to co-culture with hy926 cells.