Figure 2. Effect of MC3181 on the adhesion of human melanoma cells to different ECM components.

WM115 cells and WM266.4 cells (3×10⁵/ml) were applied to individual coated wells with 200 μl of a, d. collagen (7.5 μg/ml), b, e. gelatin (0.1%) or c, f. Matrigel (0.2 mg/ml), in the absence and in the presence of increasing MC3181 concentrations, and incubated for 30 minutes at 37°C in 5% CO₂. Cells were then fixed and colored with crystal violet. Images (20X magnification, 3X digital magnification) were obtained by phase contrast microscopy. The absorbance values of wells were measured at 580 nm after dissolving crystal violet with 100% methanol. The results were expressed as the mean percentage of cell adhesion ± SD versus control, repeated in triplicate; *P < 0.05, **P < 0.005 and *** P < 0.0005 vs control.