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. 2017 Jan 17;8(9):15520–15538. doi: 10.18632/oncotarget.14690

Figure 5. RT-PCR analysis of the mRNA expression of cancer progression related genes.

Figure 5

a. Total RNA from untreated and MC3181-treated cells was extracted, reverse-transcribed, amplified by PCR, and submitted to electrophoresis on a 1.8% agarose gel (see “Materials and Methods” section). The graphs show the relative intensity of the PCR products vs β2-microglobulin, obtained by the ImageJ analysis software: -■-ctrl; -◇- 0.05 and 0.04 μM MC3181 (WM115 and WM266.4, respectively); -●- 0.26 and 0.20 μM MC3181 (WM115 and WM266.4, respectively) and -△- 1.3 and 1.0 μM MC3181 (WM115 and WM266.4, respectively). b. Relative intensity of the PCR products from WM266.4 cells treated for 48 hours with equiactive concentrations of MC3181 and VMF (1.0 μM vs 0.9 μM, respectively).