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. 2017 Feb 2;8(9):15732–15743. doi: 10.18632/oncotarget.14997

Figure 5. Profile of expression of SerpinB3, COX-2 and β-Catenin in cell lines.

Figure 5

A. In HTC15, HT29, HepG2 and HepG2/SerpinB3 cell lines SerpinB3, COX-2, and β-Catenin transcripts were quantified at 24, 48 and 72 hours after plating by real-time PCR. Results are expressed as mean +/− SE of three independent experiments. SerpinB3: HTC15 cell line 24hr versus 72hr *p = 0.0376 and 48hr versus 72hr *p = 0.0376; HT29 cell line 24hr versus 48hr **p = 0.0018, 24hr versus 72hr *p = 0.0181 and 48hr versus 72hr *p = 0.0134; HepG2/SerpinB3 cell line 24hr versus 48hr ***p < 0.0001, 24hr versus 72hr **p = 0.0014 and 48hr versus 72hr **p = 0.0028. COX-2: HT29 cell line 24hr versus 48hr ▪▪p = 0.0098, 24h versus 72h ▪p = 0.0316 and 48h versus 72h ▪p = 0.0103; HepG2 cell line 24h versus 72hr ▪p = 0.0180; HepG2/SerpinB3 cell line 24hr versus 48hr ▪▪▪p = 0.0002, 24hr versus 72hr ▪▪p = 0.0023. β-Catenin: HTC15 cell line 24hr versus 72hr ·p = 0.0421; HT29 cell line 24hr versus 48hr ··p = 0.0047, 24hr versus 72hr ··p = 0.0065 and 48hr versus 72hr ·p = 0.0201; HepG2 cell line 24hr versus 72hr ·p = 0.0300; HepG2/SerpinB3 cell line 24hr versus 48hr ···p = 0.0004 and 48hr versus 72hr ··p = 0.0024. B. SerpinB3 (45 KDa), COX-2 (60 KDa) and β-Catenin (92 KDa) proteins were analyzed by Western blot and C. Their levels were estimated by densitometric analysis (QuantityOne software, Biorad). β-Actin expression was used for sample normalization. Results are representative of three independent experiments.