Fig. 2.

TSP1 domain of CCN3 interacts with periostin. a Domain structures of intact and domain deletion forms of CCN3 protein. All the forms were expressed as HA or FLAG tagged proteins with the tag at their C-terminal. The signal sequence from IgG was fused to the N-terminal deletion constructs (CCN3-d2 and CCN3-d4). The numbers denote the positions of the amino acids of the CCN3 protein. SS, signal sequence; IGFBP, insulin-like growth factor binding protein-like domain; VWC, von Willebrand factor type C-like domain; TSP1, thrombospondin type 1-like domain; CT, C-terminal domain. b Co-immunoprecipitation assay for ΔEMIΔCT-FLAG and HA-tagged CCN3-d2, CCN3-d4, or CCN3-d5. 293 T cells were co-transfected with the expression vectors. The total cell lysates were co-immunoprecipitated with anti-FLAG Agarose