Table 1.
Antibodies used in this study
| Antibody | Cat no. | Supplier | Host/clonality | Dilution |
|---|---|---|---|---|
| FLAG | A4596 | Sigma-Aldrich | mouse monoclonal | 1:2000 (agarose beads, IP) |
| FLAG | F7425 | Sigma-Aldrich | rabbit polyclonal | 1:2000 (W) |
| GFP | 598 | MBL | rabbit polyclonal | 1:3000 (W) |
| HA | A2095 | Sigma-Aldrich | mouse monoclonal | 1:2000 (agarose beads, IP) |
| HA | H6908 | Sigma-Aldrich | rabbit polyclonal | 1:2000 (W) |
| mPeriostin (RD1) | N/A | N/A (raised in our laboratory) | rabbit polyclonal | 1:100 (IHC) |
| mPeriostin (CT) | N/A | N/A (raised in our laboratory) | rabbit polyclonal | 1:2000 (W) |
| hCCN3 | AF1640 | R&D | goat polyclonal | 1:200 (IHC) |
| hCCN3 (K19 M) | N/A | N/A (gift from Dr. B. Perbal) | rabbit polyclonal | 1:150 (IHC) |
W Western blotting, IHC Immunohistochemistry, IP Immunoprecipitation. N/A indicates not applicable