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Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of purified esterase H8 and the substrate specificity of H8. (A) SDS-PAGE analysis of purified H8. Lane 1, purified H8; lane M, protein mass markers. (B) Substrate specificity of H8 evaluated with pNP esters. The graph shows data from triplicate experiments (mean ± SD).
