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. 2017 Mar 23;8:441. doi: 10.3389/fmicb.2017.00441

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Copyright © 2017 Zhang, Hao, Zhang, Chen, Xie, Shi, Zhou, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative specific activities and circular dichroism (CD) spectra of WT H8 and its mutants. (A) Relative specific activities of WT H8 and its mutants. The specific activity of WT H8 (69.7 U/mg) was defined as 100%. (B) CD spectra of WT H8 and its mutants. All the spectra were collected from 200 to 250 nm at a scanning speed of 200 nm/min with a path length of 0.1 cm. The graphs show data from triplicate experiments (mean ± SD).