Table 1.
Effects of metal ions and potential inhibitors on H8 activity.
| Compound | Relative/Residual activity (%) |
|
|---|---|---|
| 1 mM | 10 mM | |
| K+ | 107.8 ± 5.8 | 107.6 ± 1.5 |
| Li+ | 99.8 ± 2.1 | 86.1 ± 3.1 |
| Ba2+ | 93.4 ± 4.4 | 59.4 ± 2.8 |
| Ca2+ | 94.9 ± 8.1 | 90.9 ± 6.1 |
| Co2+ | 84.9 ± 0.8 | 74.3 ± 2.6 |
| Cu2+ | 82.2 ± 2.7 | 0.7 ± 0.2 |
| Fe2+ | 85.0 ± 1.4 | LDa |
| Mg2+ | 96.8 ± 6.1 | 84.1 ± 5.4 |
| Mn2+ | 76.0 ± 3.3 | 57.2 ± 2.6 |
| Ni2+ | 67.5 ± 1.8 | 57.1 ± 1.7 |
| Zn2+ | 25.1 ± 0.6 | 11.5 ± 0.4 |
| β-Mercaptoethanol | 78.6 ± 3.1 | 11.9 ± 2.8 |
| DTT | 31.3 ± 1.2 | 5.0 ± 2.7 |
| Thiourea | 111.5 ± 1.2 | 118.2 ± 5.1 |
| Urea | 103.5 ± 8.0 | 115.2 ± 4.0 |
| EDTA | 106.8 ± 1.8 | 114.4 ± 1.3 |
| PMSF | 96.2 ± 4.7 | 33.4 ± 1.1 |
aLD indicates that the value was less than the limit of detection.