Table 5.
Impact of mutations on H8 stability and activity.
| Enzyme | Topt (°C) | Vmax (μM/min/mg) | Km (mM) | kcat (s-1) | kcat/Km (mM-1 s-1) |
|---|---|---|---|---|---|
| WT | 35 | 73.36 ± 1.91 | 0.074 ± 0.014 | 40.10 ± 1.04 | 539.8 (100%) |
| R195E | 30 | 51.96 ± 2.92 | 0.052 ± 0.011 | 28.41 ± 1.59 | 546.2 (101.2%) |
| R203E | 30 | 8.50 ± 1.09 | 0.070 ± 0.002 | 4.64 ± 0.59 | 66.6 (12.3%) |
| R216E | 35 | 74.49 ± 2.63 | 0.072 ± 0.008 | 40.72 ± 2.44 | 546.7 (101.3%) |
| R236E | 30 | 46.53 ± 1.54 | 0.053 ± 0.003 | 25.44 ± 0.84 | 481.8 (89.3%) |
| R263E | 35 | 50.01 ± 2.37 | 0.058 ± 0.008 | 26.64 ± 1.19 | 459.3 (85.1%) |