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. 2017 Mar 23;8:323. doi: 10.3389/fimmu.2017.00323

Figure 1.

Figure 1

CXCL13 expression in subpopulation of liver cells and its regulation by toll-like receptor (TLR) ligands. (A) Comparison of CXCL13 mRNA expression in Kupffer cell (KC), hepatic stellate cell (HSC), liver sinusoidal endothelial cell (LSEC), and primary human hepatocyte (PHH). Different subpopulation of liver cells were isolated from non-hepatitis B virus-infected livers. The total RNA of these cells was extracted and the expression of CXCL13 mRNA was determined by real-time RT-PCR. (B) Comparison of CXCL13 mRNA expression in KC after stimulation with TLR1–9 ligands. Isolated KC were stimulated with different TLR1–9 ligands for 6 h and then harvested for RNA extraction. CXCL13 mRNA expression was determined by real-time RT-PCR. The stimulus and concentration used as below: TLR1/2 agonist: Pam3CSK4 (4 μg/ml), TLR2 agonist: HKLM (108 cells/ml), TLR3 agonist: poly I:C (50 μg/ml), TLR4 agonist: LPS (30 μg/ml), TLR5 agonist: FLA (2 μg/ml), TLR6/2 agonist: FSL1 (1 μg/ml), TLR7 agonist: imiquimod (20 μg/ml), TLR8 agonist: ssRNA40 (10 μg/ml), TLR9 agonist: CpG (31.8 μg/ml). Data are representative with mean ± SEM, and determined by Mann–Whitney U test.