Table 1.
List of proteins identified by ESI-MS/MS from P. ostreatus growing in lignocellulose.
| Spot no. | Biological process | JGI ID | Description | MW(kDa)/pI | Mascot score | Fold change(treat/control) | ||
|---|---|---|---|---|---|---|---|---|
| Lignin | Xylan | CMC | ||||||
| 3 | Carbohydrate metabolism and energy metabolism | jgi|1043453 | Mannose-6-phosphate isomerase | 32.9/3.91 | 98 | a | b | a |
| 99 | jgi|1036636 | Phosphatidylserine decarboxylase proenzyme 2 | 54.4/6.19 | 85 | 0.4 | 0.64 | 2.18 | |
| 109 | jgi|1070334 | Pyruvate kinase 1 | 61.6/7.47 | 151 | 0.25 | 0.75 | 1.53 | |
| 100 | jgi|1011623 | Phosphoglycerate kinase | 41.8/3.67 | 169 | 0.95 | a | 1.95 | |
| 76 | jgi|1109049 | Triosephosphate isomerase | 27.4/6.85 | 109 | 0.48 | 2.03 | 2.67 | |
| 106 | jgi|1070334 | Pyruvate kinase 1 | 45.6/7.69 | 84 | 0.47 | 1.53 | 3.16 | |
| 95 | jgi|1032066 | Phosphoglycerate kinase | 45.0/3.44 | 115 | a | 0.49 | 4.25 | |
| 86 | jgi|1019376 | Xylulose kinase | 28.9/4.01 | 87 | a | b | a | |
| 93 | jgi|1102061 | NADPH-dependent D-xylose reductase | 34.7/6.69 | 99 | 1.22 | 6.41 | 3.1 | |
| 96 | jgi|1114405 | 6-phosphogluconate dehydrogenase, decarboxylating | 62.3/3.98 | 75 | 0.3 | 0.37 | 1.54 | |
| 55 | jgi|1068318 | Ribose-5-phosphate isomerase | 31.4/6.08 | 90 | 0.34 | 1.59 | a | |
| 69 | jgi|1109004 | 6-phosphogluconate dehydrogenase | 53.7/6.23 | 147 | 0.14 | 0.17 | 0.69 | |
| 81 | jgi|1108563 | Glucose-6-phosphate dehydrogenase | 16.4/7.89 | 106 | 0.42 | a | 1.14 | |
| 75 | jgi|1090672 | Glyceraldehyde-3-phosphate dehydrogenase | 34.6/6.63 | 135 | 0.44 | 0.72 | 1.51 | |
| 77 | jgi|1037028 | Phosphogluconate dehydrogenase | 26.6/7.02 | 126 | 0.11 | 2.62 | 3.55 | |
| 54 | jgi|1101876 | Ribulose-phosphate 3-epimerase | 25.9/5.5 | 219 | 0.13 | 1.17 | 1.26 | |
| 38 | Carbohydrate metabolism and energy metabolism | jgi|1018327 | Glycosyl transferase family 4 | 81.8/6.11 | 76 | 0.514 | 0.88 | 1.13 |
| 71 | jgi|1096444 | Glucose-6-phosphate 1-dehydrogenase | 58.5/6.55 | 140 | a | 0.14 | 3.85 | |
| 70 | jgi|1064981 | Glucose-1-phosphate uridylyltransferase | 58.7/6.26 | 154 | 0.43 | 0.44 | 1.23 | |
| 72 | jgi|48499 | Pyruvate dehydrogenase | 44.9/7.7 | 163 | 0.41 | 0.57 | 0.67 | |
| 73 | jgi|48714 | Isocitrate dehydrogenase | 47.5/5.93 | 209 | 0.13 | 1.11 | 0.72 | |
| 56 | jgi|1094663 | Malate dehydrogenase | 34.2/6.12 | 139 | 0.3 | 0.35 | 1.23 | |
| 60 | jgi|1075656 | Malate dehydrogenase | 34.1/6.09 | 91 | 0.34 | 2.06 | 1.87 | |
| 23 | jgi|1097340 | Pyruvate carboxylase 2 | 18.0/4.34 | 87 | a | 7.57 | 2.16 | |
| 78 | jgi|1113799 | Rhamnogalacturonan acetylesterase | 25.6/6.02 | 181 | a | a | a | |
| 94 | jgi|1082594 | Carbon catabolite-derepressing protein kinase | 35.0/7.45 | 89 | a | 4.82 | 0.96 | |
| 36 | jgi|1053961 | ATPase | 100.3/5.27 | 239 | 0.23 | 2.58 | 1.15 | |
| 40 | jgi|1044485 | Hydrogen-transporting ATPase | 57.0/5.34 | 204 | a | 0.77 | 1.25 | |
| 115 | jgi|1089099 | Glutamine synthetase | 22.8/6.98 | 148 | a | 1.3 | 2.13 | |
| 15 | jgi|1087999 | Adenylate kinase | 27.7/6.77 | 104 | 6.29 | 2.33 | 2.26 | |
| 13 | Nucleotide metabolism | jgi|1105829 | GDP-mannose transporter | 23.7/4.26 | 92 | a | b | a |
| 88 | jgi|1099408 | Suppressor of kinetochore protein 1 | 17.4/3.63 | 76 | a | b | a | |
| 84 | jgi|47938 | Alpha-1,3/1,6-mannosyltransferase ALG2 | 49.7/4.09 | 64 | 0.45 | 2.35 | a | |
| 79 | Nucleotide metabolism | jgi|1037108 | Scavenger mRNA decapping enzyme | 25.4/6.11 | 204 | 0.19 | a | a |
| 46 | jgi|1054232 | Ribonuclease T2 | 41.5/5.92 | 198 | 0.17 | 0.27 | 0.62 | |
| 47 | jgi|1083505 | 40S ribosomal protein | 32.1/5.21 | 72 | 0.38 | 0.15 | 0.94 | |
| 41 | jgi|1056351 | cysteine-type endopeptidase | 55.0/5.81 | 88 | a | a | 1.19 | |
| 44 | jgi|1088444 | GTP binding | 42.0/5.11 | 109 | a | 1.31 | 1.23 | |
| 57 | jgi|1037683 | Ribosomal | 33.6/5.99 | 95 | 0.3 | 0.92 | 1.23 | |
| 68 | jgi|1054296 | GTPase | 93.4/6.27 | 136 | 0.28 | 0.22 | 1.4 | |
| 103 | jgi|1090777 | Glucosamine 6-phosphate N-acetyltransferase | 30.9/3.91 | 99 | 0.45 | 0.45 | 1.55 | |
| 59 | jgi|1095212 | Endo/exonuclease | 34.7/5.25 | 92 | 0.33 | 0.76 | 1.76 | |
| 113 | jgi|1106249 | Tethering factor for nuclear proteasome sts1 | 24.0/8.28 | 88 | 2.23 | 1.28 | 2.16 | |
| 101 | jgi|1075990 | cAMP-dependent protein kinase regulatory subunit | 34.1/6.25 | 78 | a | 1.63 | 2.19 | |
| 108 | jgi|1101333 | 40S ribosomal protein S29 | 45.2/7.36 | 144 | 0.46 | 0.61 | 2.63 | |
| 24 | jgi|1066340 | 60S ribosomal protein L43 | 14.7/4.98 | 106 | 2.15 | 7.78 | 3.34 | |
| 27 | jgi|1047882 | Dimethyl adenosine transferase | 36.1/9.0 | 79 | 2.32 | 0.464 | 4.64 | |
| 90 | jgi|185993 | 60S ribosomal protein L23-B | 14.3/4.26 | 83 | b | b | b | |
| 89 | Other metabolism | jgi|1114368 | Calmodulin | 31.3/3.57 | 100 | a | b | a |
| 63 | jgi|52279 | Formamidase | 42.9/5.22 | 137 | 0.3 | 0.67 | 0.93 | |
| 39 | jgi|1098883 | Amidase | 55.7/5.09 | 188 | 0.27 | a | 1.12 | |
| 64 | jgi|1093313 | Delta-aminolevulinic acid dehydratase | 35.8/5.79 | 151 | a | 1.03 | 1.52 | |
| 65 | jgi|1054502 | Enolase | 47.1/5.55 | 157 | 0.15 | 1.17 | 1.74 | |
| 66 | jgi|1107810 | CoA- transferase | 41.8/5.93 | 218 | 0.28 | 1.21 | 1.99 | |
| 97 | jgi|155235 | Mitochondrial import inner membrane translocase | 115.6/4.7 | 100 | a | 0.44 | 2.26 | |
| 61 | jgi|1067896 | Putative hydrolase | 40.5/6.32 | 157 | 0.47 | 1.74 | 2.65 | |
| 25 | jgi|1065645 | Putative Sugar transporter | 57.5/3.97 | 83 | 2.31 | 21.19 | 4.62 | |
| 114 | jgi|1094266 | Diphosphoinositol polyphosphate phosphohydrolase | 20.7/4.77 | 82 | 1.76 | 4.91 | 4.86 | |
| 35 | jgi|1107810 | Putative CoA-transferase | 41.9/5.93 | 256 | 4.29 | 4.78 | 8.18 | |
| 102 | jgi|1101425 | Sterol 3-beta-glucosyltransferase | 32.6/6.24 | 86 | b | b | b | |
| 83 | Protein and amino acid synthesis | jgi|1089644 | Leucine carboxyl methyltransferase | 50.0/3.47 | 76 | a | b | a |
| 45 | jgi|1076233 | 3-isopropylmalate dehydrogenase | 40.7/5.56 | 182 | a | 0.39 | 0.67 | |
| 48 | jgi|176309 | Glucosamine-6-phosphate isomerase | 32.8/5.81 | 136 | 0.47 | a | 0.68 | |
| 74 | jgi|1094925 | Adenosylhomocysteinase | 47.3/5.93 | 141 | a | 1.3 | 0.85 | |
| 111 | jgi|1010364 | SWI5-dependent HO expression protein 3 | 60.2/7.74 | 95 | a | a | 0.88 | |
| 37 | jgi|1091537 | Alanine–tRNA ligase | 106.3/5.52 | 116 | a | 2.54 | 1.21 | |
| 49 | jgi|1099302 | Methyltransferase | 31.0/5.7 | 206 | 0.37 | a | 1.23 | |
| 67 | jgi|1060865 | Aspartate aminotransferase | 45.4/6.54 | 176 | 0.21 | 0.37 | 1.28 | |
| 107 | jgi|1112899 | Phenylalanine ammonia-lyase | 33.4/7.08 | 96 | a | 0.75 | 1.59 | |
| 104 | jgi|1027245 | E3 ubiquitin-protein ligase TOM1 | 31.6/4.64 | 102 | 1.21 | 1.18 | 2.17 | |
| 110 | jgi|1039251 | Serine/threonine-protein kinase MEC1 | 61.4/7.65 | 79 | 2 | 0.53 | 2.57 | |
| 98 | jgi|48252 | Glycylpeptide N-tetradecanoyltransferase | 85.2/6.66 | 80 | a | a | b | |
| 22 | Redox processes | jgi|1066477 | Putative oxidoreductase | 22.8/3.22 | 72 | b | a | a |
| 10 | jgi|1027050 | Cytochrome c oxidase copper chaperone | 13.9/4.93 | 74 | 2.55 | 0.51 | 0.26 | |
| 112 | jgi|1098138 | Aldehyde dehydrogenase | 21.9/6.37 | 94 | 0.37 | 0.32 | 1.49 | |
| 34 | jgi|1045076 | Putative oxidoreductase | 32.8/5.56 | 147 | 2.24 | 2.24 | 1.79 | |
| 58 | jgi|1081301 | Putative Glucose/ribitol dehydrogenase | 33.1/6.17 | 184 | 0.42 | 1.42 | 1.89 | |
| 105 | jgi|1029362 | Isocitrate dehydrogenase [NAD] subunit 1 | 28.3/6.19 | 90 | 1.68 | 1.63 | 2.1 | |
| 31 | jgi|1086766 | Putative oxidoreductase | 26.3/5.69 | 146 | 4.24 | 2.97 | 2.24 | |
| 2 | jgi|19749 | Cytochrome c oxidase assembly protein | 31.1/3.55 | 108 | 2.98 | 2.19 | 2.32 | |
| 8 | jgi|1039979 | 3-isopropylmalate dehydrogenase | 15.5/6.24 | 82 | 3.26 | 6.52 | 2.6 | |
| 85 | jgi|1098138 | Potassium-activated aldehyde dehydrogenase | 35.4/4.65 | 63 | a | 3.38 | 3.24 | |
| 21 | jgi|1015418 | 3-hydroxyanthranilate 3,4-dioxygenase | 18.6/4.14 | 80 | 2.67 | 4.22 | 3.76 | |
| 14 | jgi|1030848 | Putative aryl-alcohol dehydrogenase | 31.4/4.95 | 95 | 9.25 | 11.13 | 4.63 | |
| 33 | jgi|1076970 | Putative oxidoreductase | 32.3/5.5 | 133 | b | b | b | |
| 5 | jgi|1015750 | Cytochrome c oxidase assembly protein 3 | 14.6/4.65 | 88 | a | a | a | |
| 16 | jgi|1087944 | Putative nitronate monooxygenase | 19.2/7.34 | 69 | b | b | b | |
| 29 | Stress response | jgi|1064479 | 14-3-3 protein homolog | 23.0/8.26 | 109 | 2.37 | a | a |
| 30 | jgi|1077356 | Glutathione-S-Trfase | 26.9/5.77 | 131 | 2.53 | 0.52 | 0.78 | |
| 9 | jgi|1077250 | Oxidation resistance protein 1 | 14.4/5.76 | 105 | 3.52 | 0.69 | 0.84 | |
| 11 | jgi|185767 | 10 kDa heat shock protein, mitochondrial | 14.4/5.76 | 64 | 4.23 | 1.26 | 0.85 | |
| 28 | jgi|1113505 | Superoxide dismutase [Cu-Zn] | 28.1/9.0 | 100 | 2.6 | 0.52 | 1.38 | |
| 87 | jgi|1022101 | Thiamine thiazole synthase | 22.8/3.52 | 79 | 0.42 | 2.19 | 1.66 | |
| 62 | jgi|1058013 | Glutathione-S-Trfase_C-like | 42.1/6.0 | 98 | a | 1.38 | 2.56 | |
| 19 | jgi|1108100 | Inheritance of peroxisomes protein 1 | 40.0/5.42 | 71 | 5.78 | 4.56 | 4.79 | |
| 6 | jgi|1032742 | Oxidant-induced cell-cycle arrest protein 5 | 16.6/3.58 | 97 | b | b | b | |
| 7 | jgi|1025559 | Monothiol glutaredoxin-5 | 13.5/4.34 | 100 | b | b | b | |
| 20 | jgi|1106868 | Alternative oxidase, mitochondrial | 45.4/4.61 | 90 | b | b | b | |
| 91 | Unkown function | jgi|1048439 | Uncharacterized protein C6B12.14c | 15.4/4.17 | 111 | a | b | 0 |
| 50 | jgi|1080037 | PLC-like phosphodiesterase | 32.7/5.0 | 89 | 0.14 | 0.2 | 0.41 | |
| 26 | jgi|1022166 | Purine phosphorylase | 43.7/7.33 | 120 | 2.32 | 2.78 | 0.7 | |
| 51 | jgi|1097135 | Calcium ion binding protein | 25.4/5.32 | 194 | a | 0.7 | 1.23 | |
| 43 | jgi|1048986 | Predicate protein | 46.5/5.34 | 182 | a | a | 1.24 | |
| 80 | jgi|1016239 | Putative protein | 15.2/5.95 | 122 | a | 1.14 | 1.29 | |
| 53 | jgi|1081199 | Putative protein | 16.8/5.71 | 94 | a | 0.93 | 1.33 | |
| 18 | jgi|1046446 | Hypothetical protein | 14.7/9.0 | 84 | 5.44 | 1.41 | 1.47 | |
| 1 | jgi|1075945 | Hypothetical protein | 34.9/3.58 | 69 | 4.47 | 2.77 | 1.52 | |
| 17 | jgi|1012191 | Hypothetical protein | 15.4/7.66 | 99 | 3.26 | 4.08 | 3.98 | |
| 32 | jgi|1107762 | Hypothetical protein | 30.0/5.38 | 192 | 9.22 | 1.84 | 4.61 | |
| 92 | jgi|176718 | Putative uncharacterized protein YKL131W | 18.8/4.76 | 141 | 1.18 | 5.54 | 6.36 | |
| 52 | jgi|1033638 | Putative protein | 21.1/4.68 | 91 | 0.48 | 26.11 | 11.14 | |
| 82 | jgi|1048248 | Bifunctional lycopene cyclase/phytoene synthase | 114.5/3.34 | 96 | a | b | b | |
| 4 | jgi|166436 | Hypothetical protein | 18.7/3.64 | 98 | b | b | b | |
| 12 | jgi|174389 | Hypothetical protein | 17.1/5.76 | 104 | b | b | b | |
a, particularly low; b, particularly high.