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. 2017 Apr;24(4):145–152. doi: 10.1101/lm.043000.116

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© 2017 Criado-Marrero et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first 12 months after the full-issue publication date (see http://learnmem.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Knockdown of FKBP5 protein with FKBP5-shRNA plasmid infusions. (A) Localized expression of GFP in IL 5 d after bilateral infusion of Fkbp5-shRNA plasmid. (B) Expression of GFP from Fkbp5-shRNA plasmid in IL neurons. (C) FKBP5 protein from IL tissue punches extracted 2 or 5 d after infusion of Fkbp5–shRNA plasmids normalized to GAPDH. (D) Western blot showing reduced expression of FKBP5 in IL tissue punches from rats 5 d after infusion of scramble or Fkbp5-shRNA plasmids. (*) P < 0.05, (**) P < 0.01, n = 4–5 rats in each group. (CC) corpus callosum.