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. 2017 Apr;24(4):153–157. doi: 10.1101/lm.044578.116

Figure 2.

Figure 2.

PSI regulates the duration of Arc expression in the hippocampal DG/CA3 and CA1 regions. (A) Experimental design. Mice (n = 10–12 per group) were placed in a novel context for 30, 180, or 720 sec prior to footshock. Naïve home cage controls were included as comparators. Hippocampal DG/CA3 and CA1 fractions were collected post-conditioning and Arc and β-actin immunoreactivity were assessed. (B–G) Time-courses of Arc immunoreactivity in the DG/CA3 and CA1 following conditioning at the 30, 180, or 720 sec PSI. (B,C) 30 sec PSI. Arc activity was increased at 60 min post-conditioning in the DG/CA3 (B) but not in the CA1 (C). (D,E) 180 sec PSI. Arc activity was increased at 60 and 120 min post-conditioning in the DG/CA3 (D) and the CA1 (E). (F,G) 720 sec PSI. Arc activity showed a notable increase at 60–360 min post-conditioning in the DG/CA3 (F) and 60–120 min in the CA1 (G). Images for figures C and E were spliced together from different sections of the same membrane for convenient viewing. Data were collected from three experiments with similar results. Data are presented as mean + SE. Significant difference from home cage controls at each time-point is indicated by (*) P < 0.05, (**) P < 0.01, (***) P < 0.001.