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. 2017 Mar 23;7:45090. doi: 10.1038/srep45090

Figure 1. Effect of bursectomy on B and T cell populations.

Figure 1

B cell depletion in young chicks was achieved by the chemical bursectomy method using cyclophosphamide. To assess the effect of the treatment on B and T cell populations, blood samples from birds were taken at 3, 5 and 7 weeks of age (day 0 and 14 and 28 days post infection respectively). The Bu-1 marker was used to identify avian B lymphocytes (A) and CD4, CDα and CD8β markers were used for T lymphocytes (B–D). Bars represent the median values; circles represent individual birds. At each time point there were 10–12 birds per group. Statistically significant differences were only observed in the B cell population. **p ≤ 0.01; ***p ≤ 0.001; NS = non-significant.